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P27 antibody

Reactivity: Human IP, ELISA, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN233757
$537.90
Plus shipping costs $45.00 and $20.00 dry ice
100 μL
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  • Target
    P27
    Reactivity
    • 22
    • 8
    • 8
    • 1
    • 1
    Human
    Host
    • 22
    • 1
    Rabbit
    Clonality
    • 22
    • 1
    Polyclonal
    Conjugate
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 13
    • 11
    • 6
    • 4
    • 1
    • 1
    Immunoprecipitation (IP), ELISA, Western Blotting (WB)
    Characteristics
    Concentration Definition: by Refractometry
    Purification
    Antiserum
    Immunogen
    This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27 protein.
    Immunogentype:Recombinant
  • Application Notes
    This antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immmunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins. Optimal titers for other applications should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    80 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Target
    P27
    Synonyms
    Kip1 antibody, p27 antibody, cyclin dependent kinase inhibitor 1B antibody, CDKN1B antibody
    Target Type
    Viral Protein
    Background
    P27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21.  p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
    Synonyms: Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1
    Gene ID
    1027, 4757962
    UniProt
    P46527
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