Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Cross-Reactivity
Mouse (Murine), Rat (Rattus)
Characteristics
Concentration Definition: by UV absorbance at 280 nm
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
STAT5A
Reactivity: Human, Mouse, Rat, Cow
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Target
STAT5A
(Signal Transducer and Activator of Transcription 5A (STAT5A))
MGF antibody, STAT5 antibody, AA959963 antibody, Stat5 antibody, STAT5A/MGF antibody, STATA5 antibody, STAT5A antibody, stat5 antibody, stat5b antibody, signal transducer and activator of transcription 5A antibody, signal transducer and activator of transcription 5a antibody, STAT5A antibody, Stat5a antibody, stat5a antibody, LOC100348518 antibody, LOC100716811 antibody
Background
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Signal trans-ducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic trans-cription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5b. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Synonyms: MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5A antibody, Transcription factor STAT5A antibody