APOE antibody (Apolipoprotein E) (HRP)

Details for Product anti-APOE Antibody No. ABIN236820
Antigen
Reactivity
Human
271
79
48
5
5
2
2
2
2
1
1
Host
Goat
170
80
67
1
Clonality
Polyclonal
Conjugate
This APOE antibody is conjugated to HRP
28
14
14
5
5
5
5
5
4
3
3
3
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
226
172
86
53
19
18
16
15
12
12
6
5
4
3
3
2
2
2
1
1
1
1
Options
Immunogen Purified human Apo E from human plasma
Specificity Binds to human Apo E.
Characteristics Goat anti Apolipoprotein E, Goat antibody to Human Apolipoprotein E (Apo E) Horseradish Peroxidase conjugated
Purification Human Apo E-Sepharose affinity column, and conjugated with HRP
Antigen
Alternative Name Apolipoprotein E (Apo E) (APOE Antibody Abstract)
Pathways Regulation of Cell Size, Lipid Metabolism
Application Notes Can be used for immunoblot (1:100-1:2.000) and ELISA (1:2.000-1:8.000). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 0.5 mL with distilled water.
Buffer Lyophilized from 50 mM PBS, 0.1 M Sodium chloride, pH 7.4 containing 10 mg/mL BSA, 0.01 % Thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Prepare working dilution only prior to immediate use. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Centrifuge product if not completely clear after standing for 1-2 hours at room temperature.
Storage 4 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50 % followed by storage at -28 °C.
Expiry Date 12 months