Mouse anti-Cow IgG Antibody

Catalog No. ABIN238423

Product Details

Target: IgG

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Species reactivity (tested):Bovine.

Purification: Protein A affinity chromatography

Immunogen: Fraction of Bovine immunoglobulin.

Clone: IVA285-1

Isotype: IgG1

Application Details

Application Notes: Western Blotting (Reducing conditions): 0,5-1 μg/mL, overnight in 4C. SDS-PAGE (12 % separating gel). Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmlireducing buffer, boiled in water bath for 3 min. Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry (frozen sections).

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Bovine Immunoglobulin G