CD14 antibody (Biotin)

Catalog No. ABIN238455

Product Details anti-CD14 Antibody (Biotin)

Target: CD14

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates.

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria

Clone: MEM-18

Isotype: IgG1

Application Details

Application Notes: Suitable for indirect Immunofluorescence analysis by Flow Cytometry: Suggested working dilution is 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze/thaw cycles.
Shelf Life: One year from despatch.

Expiry Date: 12 months

References for anti-CD14 antibody (Biotin) (ABIN238455)

Weiss, Lichtenauer, Kirchner, Stock, Aurich, Christ, Brockhoff, Kunz-Schughart, Jauch, Schlitt, Thasler: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Iwaki, Nishitani, Mitsuzawa, Hyakushima, Sano, Kuroki: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, (2005) (PubMed).

Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).

Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, (2001) (PubMed).

Bazil, Baudys, Hilgert, Stefanová, Low, Zbrozek, Horejsí: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, (1989) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades