XRCC5 antibody

Catalog No. ABIN238473

Product Details anti-XRCC5 Antibody

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC5 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: The antibody MEM-54 reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.

Characteristics: Synonyms: TLAA, G22P2, CTCBF, CTC85, DNA repair protein XRCC5, ATP-dependent DNA helicase II 80kDa subunit, Lupus Ku autoantigen protein p86, 86 kDa subunit of Ku antigen,Thyroid-lupus autoantigen, CTC box-binding factor 85 kDa subunit, Nuclear factor IV

Purification: Affinity Chromatography on Protein A

Immunogen: human whole T-lymphocytes.

Clone: MEM-54

Isotype: IgG2a

Application Details

Application Notes: Immunocytochemistry (2 μg/mL, PFA-fixation). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 containing 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for XRCC5

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Alternative Name: XRCC5 / Ku80 / Ku86 (XRCC5 Products)

Synonyms: AI314015 antibody, Ku80 antibody, Ku86 antibody, Kup80 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, NFIV antibody, xrcc5-a antibody, XRCC5 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog antibody, XRCC5 antibody, Xrcc5 antibody, xrcc5.L antibody

Background: Ku Antigen (DNA-dependent DNA helicase) is a heterodimer (of 72 and 87 kDa polypeptides) which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end-joining pathway. A DNA double-strand break is a major lesion that destroys the integrity of the DNA molecule. Such damage is introduced by ionizing radiation. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent protein kinase complex may have a role in those same processes. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle 2 full turns of DNA while encircling only the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features are well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. Mouse cells deficient for Ku80 display a marked increase in chromosomal aberrations, including breakage, translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/- mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 to promote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminated pro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends of double-stranded DNA in a cell cycle-dependent manner, being associated with chromosomes of interphase cells, followed by complete dissociation from the condensing chromosomes in early prophase. Some patients with systemic lupus erythematosus produce very large amounts of autoantibodies to p70 and p80. The autoantibody has been found in patients with autoimmune thyroid disease (Graves disease) as well as in those with lupus.Synonyms: 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, Lupus Ku autoantigen protein p86, Nuclear factor IV, TLAA, Thyroid-lupus autoantigen

Gene ID: 7520

UniProt: P13010

Pathways: DNA Damage Repair