RecA (full length) antibody
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- Target
- RecA
- Binding Specificity
- full length
- Reactivity
- E. coli
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Purification
- Affinity-purified with purified RecA protein conjugated with agarose beads.
- Sterility
- Sterile filtered
- Immunogen
- Highly purified full-length recombinant E. coli RecA protein
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- Application Notes
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1. Western blotting: 1/3,000 dilution
2. Immunoprecipitation: 1/600 dilution
3. Indirect immuno-fluorescent staining (assay dependent)
4. ELISA (assay dependent) - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS with 50 % Glycerol
- Storage
- -20 °C
- Storage Comment
- Upon receipt aliquot and store at -20 C
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- Target
- RecA
- Background
- E. coli RecA protein (352 aa, 38 kDa) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its coprotease activity after DNA damage, and induces many proteins related to DNA repair including RecA itself.
- UniProt
- P0A7G6
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