HSBP1 antibody

Catalog No. ABIN2458158

Product Details anti-HSBP1 Antibody

Target: HSBP1 (Heat Shock Factor Binding Protein 1 (HSBP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSBP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSBP1.

Application Details

Application Notes: HSBP1 antibody can be used for detection of HSBP1 by ELISA at 1:312500. HSBP1 antibody can be used for detection of HSBP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HSBP1 antibody at -20 °C.

Target Details for HSBP1

Target: HSBP1 (Heat Shock Factor Binding Protein 1 (HSBP1))

Alternative Name: HSBP1 (HSBP1 Products)

Synonyms: HSBP1 antibody, npc-a-13 antibody, NPC-A-13 antibody, hsbp1 antibody, hsbp1a antibody, 0610007A03Rik antibody, AA408769 antibody, Hsp25 antibody, zgc:73224 antibody, heat shock factor binding protein 1 like 1 antibody, heat shock factor binding protein 1 antibody, Heat shock factor binding protein 1 antibody, heat shock factor binding protein 1 S homeolog antibody, heat shock factor binding protein 1 L homeolog antibody, heat shock factor binding protein 1b antibody, HSBP1L1 antibody, HSBP1 antibody, Bm1_07370 antibody, hsbp1.S antibody, hsbp1 antibody, hsbp1.L antibody, Hsbp1 antibody, hsbp1b antibody

Background: The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.

Molecular Weight: 8 kDa

Gene ID: 3281

NCBI Accession: NP_001528

UniProt: O75506

Pathways: SARS-CoV-2 Protein Interactome