RFX1 antibody

Catalog No. ABIN2458302

Product Details anti-RFX1 Antibody

Target: RFX1 (Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFX1.

Application Details

Application Notes: RFX1 antibody can be used for detection of RFX1 by ELISA at 1:62500. RFX1 antibody can be used for detection of RFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFX1 antibody at -20 °C.

Target Details for RFX1

Target: RFX1 (Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))

Alternative Name: RFX1 (RFX1 Products)

Synonyms: EFC antibody, RFX antibody, AI047719 antibody, AI385641 antibody, zgc:162914 antibody, regulatory factor X1 antibody, regulatory factor X, 1 (influences HLA class II expression) antibody, regulatory factor X, 1a (influences HLA class II expression) antibody, RFX1 antibody, Rfx1 antibody, rfx1a antibody

Background: RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 105 kDa

Gene ID: 5989

NCBI Accession: NP_002909

UniProt: P22670