MARCH1
Reactivity: Human
ELISA
Host: Mouse
Monoclonal
unconjugated
Application Notes
MARCH1 antibody can be used for detection of 40238 by ELISA at 1:1562500. MARCH1 antibody can be used for detection of 40238 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store MARCH1 antibody at -20 °C.
Target
MARCH1
(Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))
MARCH1 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC, CD86 and FAS, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.