LMX1A antibody

Catalog No. ABIN2460346

Product Details anti-LMX1A Antibody

Target: LMX1A (LIM Homeobox Transcription Factor 1, alpha (LMX1A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LMX1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LMX1A.

Application Details

Application Notes: LMX1A antibody can be used for detection of LMX1A by ELISA at 1:62500. LMX1A antibody can be used for detection of LMX1A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store LMX1A antibody at -20 °C.

Target Details for LMX1A

Target: LMX1A (LIM Homeobox Transcription Factor 1, alpha (LMX1A))

Alternative Name: LMX1A (LMX1A Products)

Synonyms: Lmx1.1 antibody, dr antibody, dreher antibody, sst antibody, LMX1 antibody, LMX1.1 antibody, si:ch211-260g14.3 antibody, LIM homeobox transcription factor 1 alpha antibody, LIM homeobox transcription factor 1, alpha antibody, Lmx1a antibody, LMX1A antibody, lmx1a antibody

Background: Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS, MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin. Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS, MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-199 AL160058.8 14249-14447 c 200-2447 AK127724.1 362-2609 2448-3382 AL390730.12 9415-10349 c

Molecular Weight: 43 kDa

Gene ID: 4009

NCBI Accession: NP_796372

UniProt: Q8TE12

Pathways: Dopaminergic Neurogenesis