POLR2B antibody

Catalog No. ABIN2460474

Product Details anti-POLR2B Antibody

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.

Application Details

Application Notes: POLR2B antibody can be used for detection of POLR2B by ELISA at 1:312500. POLR2B antibody can be used for detection of POLR2B by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR2B antibody at -20 °C.

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: POLR2B (POLR2B Products)

Synonyms: Pol2rb antibody, Rpb140 antibody, Rpb2 antibody, POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, RNA polymerase II subunit B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, polymerase (RNA) II (DNA directed) polypeptide B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antibody, POLR2B antibody, polr2b antibody, LOC100640211 antibody, Polr2b antibody, polr2b.L antibody, rpb-2 antibody

Background: POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Molecular Weight: 134 kDa

Gene ID: 5431

NCBI Accession: NP_000929

UniProt: P30876

Pathways: Regulatory RNA Pathways, DNA Damage Repair