HIF3A antibody

Catalog No. ABIN2461997

Product Details anti-HIF3A Antibody

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HIF3A.

Application Details

Application Notes: HIF3A antibody can be used for detection of HIF3A by ELISA at 1:312500. HIF3A antibody can be used for detection of HIF3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HIF3A antibody at -20 °C.

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: HIF3A (HIF3A Products)

Synonyms: HIF-3A antibody, IPAS antibody, MOP7 antibody, PASD7 antibody, bHLHe17 antibody, Ipas antibody, NEPAS antibody, Mop7 antibody, HIF3A antibody, hypoxia inducible factor 3 alpha subunit antibody, hypoxia inducible factor 3, alpha subunit antibody, HIF3A antibody, Hif3a antibody

Background: HIF3A is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression.The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene.

Molecular Weight: 72 kDa, 66 kDa, 72 kDa

Gene ID: 64344

NCBI Accession: NP_690007

UniProt: Q9Y2N7

Pathways: Warburg Effect