PIGF antibody

Catalog No. ABIN2462126

Product Details anti-PIGF Antibody

Target: PIGF (Phosphatidylinositol Glycan F (PIGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIGF.

Application Details

Application Notes: PIGF antibody can be used for detection of PIGF by ELISA at 1:12500. PIGF antibody can be used for detection of PIGF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PIGF antibody at -20 °C.

Target Details for PIGF

Target: PIGF (Phosphatidylinositol Glycan F (PIGF))

Alternative Name: PIGF (PIGF Products)

Synonyms: pigf antibody, zgc:77184 antibody, phosphatidylinositol glycan anchor biosynthesis class F antibody, phosphatidylinositol glycan anchor biosynthesis, class F antibody, phosphatidylinositol glycan anchor biosynthesis class F S homeolog antibody, PIGF antibody, Pigf antibody, pigf.S antibody, pigf antibody

Background: PIGF is a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. PIGF and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 25 kDa

Gene ID: 5281

NCBI Accession: NP_002634

UniProt: Q07326

Pathways: Inositol Metabolic Process