EBP antibody

Catalog No. ABIN2463004

Product Details anti-EBP Antibody

Target: EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBP antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EBP.

Application Details

Application Notes: EBP antibody can be used for detection of EBP by ELISA at 1:12500. EBP antibody can be used for detection of EBP by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EBP antibody at -20 °C.

Target Details for EBP

Target: EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))

Alternative Name: EBP (EBP Products)

Synonyms: cdpx2 antibody, cho2 antibody, cpx antibody, cpxd antibody, zgc:91895 antibody, CDPX2 antibody, CHO2 antibody, CPX antibody, CPXD antibody, AI255399 antibody, Pabp antibody, Td antibody, mSI antibody, emopamil binding protein (sterol isomerase) antibody, emopamil binding protein (sterol isomerase) L homeolog antibody, phenylalkylamine Ca2+ antagonist (emopamil) binding protein antibody, Ebp antibody, ebp.L antibody, ebp antibody, EBP antibody

Background: EBP is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in EBP gene cause Chondrodysplasia punctata 2 (CDPX2, also known as Conradi-Hunermann syndrome).Emopamil-binding protein (EBP) is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. EBP shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of EBP is its high content of aromatic amino acid residues (>23 % ) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2, also known as Conradi-Hunermann syndrome).

Molecular Weight: 26 kDa

Gene ID: 10682

NCBI Accession: NP_006570

UniProt: Q15125