SPRY2 antibody

Catalog No. ABIN2463342

Product Details anti-SPRY2 Antibody

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPRY2.

Application Details

Application Notes: SPRY2 antibody can be used for detection of SPRY2 by ELISA at 1:1562500. SPRY2 antibody can be used for detection of SPRY2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SPRY2 antibody at -20 °C.

Target Details for SPRY2

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Alternative Name: SPRY2 (SPRY2 Products)

Synonyms: wu:fk66h11 antibody, wu:fk93e11 antibody, zgc:110440 antibody, hspry2 antibody, xspry2 antibody, sprouty2 antibody, Xsprouty2 antibody, sprouty-2 antibody, Xtsprouty2 antibody, hSPRY2 antibody, sprouty RTK signaling antagonist 2 antibody, sprouty RTK signaling antagonist 2 S homeolog antibody, sprouty homolog 2 (Drosophila) antibody, spry2 antibody, SPRY2 antibody, spry2.S antibody, Spry2 antibody

Background: SPRY2 is a protein belonging to the sprouty family. It contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. SPRY2 is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. SPRY2 may play a role in alveoli branching during lung development as shown by a similar mouse protein.This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 35 kDa

Gene ID: 10253

NCBI Accession: NP_005833

UniProt: O43597

Pathways: EGFR Signaling Pathway, Sensory Perception of Sound, EGFR Downregulation