MDFIC antibody

Catalog No. ABIN2463382

Product Details anti-MDFIC Antibody

Target: MDFIC (MyoD Family Inhibitor Domain Containing (MDFIC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDFIC antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MDFIC.

Application Details

Application Notes: MDFIC antibody can be used for detection of MDFIC by ELISA at 1:1562500. MDFIC antibody can be used for detection of MDFIC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MDFIC antibody at -20 °C.

Target Details for MDFIC

Target: MDFIC (MyoD Family Inhibitor Domain Containing (MDFIC))

Alternative Name: MDFIC (MDFIC Products)

Synonyms: HIC antibody, Kdt1 antibody, Mdfid antibody, MyoD family inhibitor domain containing antibody, MyoD family inhibitor domain containing S homeolog antibody, MDFIC antibody, Mdfic antibody, mdfic.S antibody

Background: MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization, p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization, p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.

Molecular Weight: 37 kDa

Gene ID: 29969

NCBI Accession: NP_951038

UniProt: Q9P1T7