CRISP1 antibody

Catalog No. ABIN2463571

Product Details anti-CRISP1 Antibody

Target: CRISP1 (Cysteine-Rich Secretory Protein 1 (CRISP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRISP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRISP1.

Application Details

Application Notes: CRISP1 antibody can be used for detection of CRISP1 by ELISA at 1:312500. CRISP1 antibody can be used for detection of CRISP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CRISP1 antibody at -20 °C.

Target Details for CRISP1

Target: CRISP1 (Cysteine-Rich Secretory Protein 1 (CRISP1))

Alternative Name: CRISP1 (CRISP1 Products)

Synonyms: AEGL1 antibody, ARP antibody, CRISP-1 antibody, HSCRISP1D antibody, HSCRISP1G antibody, HUMARP antibody, AW324342 antibody, Aeg1 antibody, AEG antibody, crisp-1 antibody, cysteine rich secretory protein 1 antibody, cysteine-rich secretory protein 1 antibody, CRISP1 antibody, Crisp1 antibody

Background: Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. CRISP1 is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface.Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.

Molecular Weight: 27 kDa

Gene ID: 167

NCBI Accession: NP_001122

UniProt: P54107