Nebulette antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Nebulette antibody was raised against a 10 amino acid synthetic peptide near the internal region of Nebulette.
NEBL
Reactivity: Human, Mouse, Rat
WB, IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 488
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 30 kDa band in human frontal cortex lysates, but not in human, mouse and rat heart lysates after 1 μg/mL antibody staining. This protein has a calculated MW of 31.2 kDa according to NP_998734.1. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.