SKA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SKA3 antibody was raised against a 13 amino acid synthetic peptide near the internal region of SKA3.
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 35 kDa and 150 kDa band in HeLa lysates after 2 μg/mL antibody staining. Both bands were successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SKA3
(Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))