This antibody is expected to recognize all reported isoforms (NP_001098547.3, NP_005232.2, NP_004982.2, NP_001157471.1, NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2
Purification
MECOM antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MECOM antibody was raised against a 10 amino acid synthetic peptide near the internal region of MECOM.
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 170 kDa band observed in A549 and HepG2 lysate (calculated MW of 126 kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.