HCN3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HCN3 antibody was raised against a 13 amino acid synthetic peptide near the internal region of HCN3.
HCN3
Reactivity: Human, Mouse, Rat
WB, IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 750
Application Notes
ELISA: Antibody detection limit dilution 1:32000 Western Blot: Approximately 85 kDa band observed in rat kidney lysates (calculated MW of 86.0 kDa according to human NP_065948.1 and 86.8 kDa according to rat NP_446137.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.