Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
DCDC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DCDC2 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DCDC2.
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approximately 52 kDa band observed (calculated MW of 52.8 kDa according to NP_057440.1). Recommmended concentration 0.1-0.3 μg/mL. IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10μg/mL. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found positive results in IHC on Rat dorsal root ganglion.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.