This product does not work on cryostat sections of Normal Human Skin.
Purification
Collagen VII antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Collagen VII antibody was raised against a 12 amino acid synthetic peptide near the internal region of Collagen VII.
COL7
Reactivity: Mouse
IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 350
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 250 kDa band observed in rat testis lysates (calculated MW of 159 kDa according to rat NP_001100328.1). Recommended concentration: 0.1-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.