This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
Purification
PNPLA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PNPLA3 antibody was raised against a 12 amino acid synthetic peptide near the internal region of PNPLA3.
PNPLA3
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 45 kDa band observed in human heart lysates (calculated MW of 42.6 kDa according to NP_473429.2). Recommended concentration: 0.03-0.1 μg/mL. Approximately 45 kDa band observed in mouse heart lysates (calculated MW of 42.6 kDa according to human NP_473429.2 and to mouse NP_473429.2). Recommended concentration: 0.05-0.2 μg/mL. Immunohistochemistry: In paraffin embedded human Skin shows membranous staining of basak kayer cells. Recommended concentration, 4-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.