This antibody is expected to recognize isoform 1 (NP_663615.1), isoform 2 (NP_055164.1, NP_085147.1, NP_663614.1) and isoform 3 (NP_663616.1, NP_663617.1).
Purification
APOL3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
APOL3 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of APOL3.
APOL3
Reactivity: Human
ELISA, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 40 kDa band observed in lysates of cell lines DAUDI and MOLT4 (calculated MW of 44.3 kDa according to NP_663615.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.