S100A9 antibody (Internal Region)

Catalog No. ABIN2466632

Product Details anti-S100A9 Antibody

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: S100A9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: S100A9 antibody was raised against a 15 amino acid synthetic peptide near the internal region of S100A9.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 16 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 13.2 kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15 kDa ) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280 (50) :41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows nuclear staining in select cells, Recommended concentration, 2-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 (S100A9 Products)

Synonyms: 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, S100A9 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody

Gene ID: 6280

NCBI Accession: NP_002956

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins