Rituximab antibody

Details for Product No. ABIN2476369
Antigen
Reactivity
Chemical
15
Host
Rat
8
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
Application
Flow Cytometry (FACS), ELISA
10
6
2
2
Options
Immunogen F(ab)2 fragment of Rituximab.
Clone MB2A4
Isotype IgG2a
Specificity Rat anti rituximab antibody, clone MB2A4, specifically recognizes the idiotypic determinants expressed by the rituximab chimeric monoclonal antibody. Rituximab is a therapeutic reagent directed against the human CD20 antigen expressed on the surface of B-cells.
Characteristics Rat anti-rituximab antibody, clone MB2A4, recognizes the monoclonal antibody rituximab, which targets CD20. This anti-rituximab antibody is highly specific and ideal for bioanalytical assay development (PK, PD, ADA assays) in preclinical R&D and clinical trials. Rat anti rituximab antibody, clone MB2A4, is specific for rituximab and does not recognize other CD20 antibodies. Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy (Cragg et al. 2004 and Hampson et al. 2010). Clone MB2A4 has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the anti-rituximab antibody to cell bound rituximab (Beum et al. 2004).
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Antigen
Target Type Chemical
Background Synonyms: MabThera ® , Rituxan®
Application Notes ELISA: 5 µg/mL as coating antibody This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP, in a sandwich ELISA together with ABIN5562178 as the capture reagent. Flow: 50 µg/mL Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer TRIS buffered saline, 0.09% Sodium Azide (NaN3)
Handling Advice Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store at +4°C or at -20°C if preferred. This product should be stored undiluted.
Product cited in: Cragg, Bayne, Illidge, Valerius, Johnson, Glennie: "Apparent modulation of CD20 by rituximab: an alternative explanation." in: Blood, Vol. 103, Issue 10, pp. 3989-90; author reply 3990-1, 2004 (PubMed).

Cragg, Bayne, Tutt, French, Beers, Glennie, Illidge: "A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells." in: Blood, Vol. 104, Issue 8, pp. 2540-2, 2004 (PubMed).

Beum, Kennedy, Li, Pawluczkowycz, Williams, Taylor: "Complement activation and C3b deposition on rituximab-opsonized cells substantially blocks binding of phycoerythrin-labeled anti-mouse IgG probes to rituximab." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 37-42, 2004 (PubMed).

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