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Ubiquitin antibody (HRP)

Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal HRP
Catalog No. ABIN2481457
  • Target See all Ubiquitin Antibodies
    Ubiquitin
    Reactivity
    • 148
    • 70
    • 70
    • 68
    • 18
    • 18
    • 16
    • 15
    • 14
    • 14
    • 14
    • 14
    • 13
    • 12
    • 11
    • 10
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    Host
    • 108
    • 72
    • 3
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 100
    • 86
    Polyclonal
    Conjugate
    • 80
    • 14
    • 13
    • 13
    • 12
    • 11
    • 7
    • 7
    • 7
    • 7
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Ubiquitin antibody is conjugated to HRP
    Application
    • 178
    • 104
    • 95
    • 69
    • 59
    • 42
    • 27
    • 21
    • 21
    • 14
    • 13
    • 10
    • 4
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificity
    Detects ~10 kDa. It also recognizes ubiquinated proteins.
    Cross-Reactivity
    Chicken, Cow, Dog, Drosophila melanogaster, Fish, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rainbow Trout, Rat, Saccharomyces cerevisiae, Salmon, Sheep, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogen
    Native bovine Ubiquitin, conjugated to KLH
    Top Product
    Discover our top product Ubiquitin Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN2481457 was sufficient for detection of free ubiquitin in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Ubiquitin
    Alternative Name
    Ubiquitin (Ubiquitin Products)
    Synonyms
    CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
    Background
    Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
    Gene ID
    281370
    NCBI Accession
    NP_776558
    UniProt
    P0CG53
    Pathways
    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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