SLC17A7 antibody (AA 493-560) (Alkaline Phosphatase (AP))

Catalog No. ABIN2483724

Product Details anti-SLC17A7 Antibody (Alkaline Phosphatase (AP))

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Binding Specificity: AA 493-560

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC17A7 antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Detects ~52 kDa. No cross-reactivity against VGlut2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Clone: S28-9

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483724 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SLC17A7

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Alternative Name: VGLUT1 (SLC17A7 Products)

Synonyms: 2900052E22Rik antibody, AI851913 antibody, VGluT1 antibody, Vglut1 antibody, BNPI antibody, VGLUT1 antibody, slc17a7 antibody, vglut1 antibody, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody, solute carrier family 17 member 7 antibody, solute carrier family 17 (vesicular glutamate transporter), member 7a antibody, Slc17a7 antibody, SLC17A7 antibody, slc17a7a antibody

Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

Gene ID: 116638

NCBI Accession: NP_446311

UniProt: Q62634