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HIF1A antibody (AA 329-530) (PerCP)

HIF1A Reactivity: Mouse WB, IHC, ELISA, IF, ICC Host: Mouse Monoclonal ESEE122 PerCP
Catalog No. ABIN2484785
  • Target See all HIF1A Antibodies
    HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
    Binding Specificity
    • 20
    • 19
    • 16
    • 16
    • 12
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 329-530
    Reactivity
    • 168
    • 85
    • 81
    • 24
    • 23
    • 20
    • 14
    • 12
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 160
    • 52
    • 6
    Mouse
    Clonality
    • 166
    • 52
    Monoclonal
    Conjugate
    • 116
    • 29
    • 18
    • 8
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HIF1A antibody is conjugated to PerCP
    Application
    • 188
    • 89
    • 75
    • 56
    • 51
    • 40
    • 39
    • 27
    • 26
    • 26
    • 12
    • 9
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~116 kDa. Specific for HIF1Alpha.
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Recombinant fragment corresponding to amino acids 329-530
    Clone
    ESEE122
    Isotype
    IgG1
    Top Product
    Discover our top product HIF1A Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2484785 was sufficient for detection of HIF1α in 20 μg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
    Alternative Name
    HIF 1 alpha (HIF1A Products)
    Synonyms
    hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody
    Background
    Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
    Gene ID
    15251
    NCBI Accession
    NP_034561
    UniProt
    Q61221
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect
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