P4HB antibody (AA 52-63) (Alkaline Phosphatase (AP))

Catalog No. ABIN2484900

Product Details anti-P4HB Antibody (Alkaline Phosphatase (AP))

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Binding Specificity: AA 52-63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P4HB antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~48 kDa.

Cross-Reactivity: Human

Purification: Protein G Purified

Immunogen: Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein

Clone: 2E7-7

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484900 was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI (P4HB Products)

Synonyms: DSI antibody, ERBA2L antibody, GIT antibody, P4Hbeta antibody, PDI antibody, PDIA1 antibody, PHDB antibody, PO4DB antibody, PO4HB antibody, PROHB antibody, PDA2 antibody, PDIP antibody, PDIR antibody, ERp59 antibody, Pdia1 antibody, Thbp antibody, p55 antibody, XPDIp antibody, pdi antibody, 1810041F13Rik antibody, AI661267 antibody, Pdip antibody, Pdipl antibody, p58 antibody, prolyl 4-hydroxylase subunit beta antibody, protein disulfide isomerase family A member 2 antibody, prolyl 4-hydroxylase, beta polypeptide antibody, protein disulfide isomerase family A member 2 L homeolog antibody, protein disulfide isomerase associated 2 antibody, prolyl 4-hydroxylase subunit beta L homeolog antibody, protein disulfide-isomerase antibody, P4HB antibody, PDIA2 antibody, P4hb antibody, pdia2.L antibody, Pdia2 antibody, p4hb.L antibody, LOC8281065 antibody

Background: Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

Gene ID: 81567

NCBI Accession: NP_001139021

UniProt: Q8NBS9

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism