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SHANK3 antibody (AA 840-857) (Alkaline Phosphatase (AP))

SHANK3 Reactivity: Rat WB, IHC, IP, IF, ICC, AA Host: Mouse Monoclonal S69 Alkaline Phosphatase (AP)
Catalog No. ABIN2485410
  • Target See all SHANK3 Antibodies
    SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
    Binding Specificity
    • 15
    • 11
    • 11
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 840-857
    Reactivity
    • 46
    • 11
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 26
    • 24
    Mouse
    Clonality
    • 26
    • 24
    Monoclonal
    Conjugate
    • 15
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SHANK3 antibody is conjugated to Alkaline Phosphatase (AP)
    Application
    • 34
    • 30
    • 24
    • 23
    • 18
    • 13
    • 13
    • 10
    • 8
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)
    Specificity
    Detects ~190 kDa. No cross-reactivity against Shank1 or Shank2.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Synthetic peptide amino acids 840-857 of rat Shank3
    Clone
    S69
    Isotype
    IgG2b
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2485410 was sufficient for detection of Shank3 in 10 μg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
    Alternative Name
    SHANK3 (SHANK3 Products)
    Synonyms
    DEL22q13.3 antibody, PROSAP2 antibody, PSAP2 antibody, SCZD15 antibody, SPANK-2 antibody, Prosap2 antibody, AI841104 antibody, Shank3b antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody, SHANK3 antibody, shank3 antibody, Shank3 antibody
    Background
    Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (2). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (3).
    Gene ID
    59312
    NCBI Accession
    NP_067708
    UniProt
    Q9JLU4
    Pathways
    Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity
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