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RAS antibody (AA 31-43) (Atto 594)

Reactivity: Human WB, IHC, IF, ICC Host: Rabbit Polyclonal Atto 594
Catalog No. ABIN2485490
  • Target See all RAS products
    RAS
    Binding Specificity
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-43
    Reactivity
    • 38
    • 19
    • 18
    • 9
    • 1
    Human
    Host
    • 37
    • 1
    Rabbit
    Clonality
    • 28
    • 10
    Polyclonal
    Conjugate
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RAS antibody is conjugated to Atto 594
    Application
    • 24
    • 11
    • 11
    • 11
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~21 kDa.
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2485490 was sufficient for detection of Ras in 20 μg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    RAS
    Abstract
    RAS Products
    Synonyms
    asr antibody, resistance to audiogenic seizures antibody, ras antibody
    Background
    The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
    Gene ID
    18176
    NCBI Accession
    NP_035067
    UniProt
    B2KGV5
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