RAS antibody (AA 31-43) (Atto 594)
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- Target See all RAS products
- RAS
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Binding Specificity
- AA 31-43
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RAS antibody is conjugated to Atto 594
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects ~21 kDa.
- Cross-Reactivity
- Cow, Human, Mouse, Rat
- Purification
- Protein G Purified
- Immunogen
- Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
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- Application Notes
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:200)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2485490 was sufficient for detection of Ras in 20 μg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- RAS
- Abstract
- RAS Products
- Synonyms
- asr antibody, resistance to audiogenic seizures antibody, ras antibody
- Background
- The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
- Gene ID
- 18176
- NCBI Accession
- NP_035067
- UniProt
- B2KGV5
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