DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody (Streptavidin)

Details for Product No. ABIN2486011
Antigen
Epitope
AA 637-650
55
49
28
25
21
18
17
16
15
15
15
15
8
7
7
7
6
6
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Fish, Human, Mouse (Murine), Zebrafish (Danio rerio)
405
159
71
53
48
4
2
2
2
1
1
1
1
1
1
Host
Mouse
302
98
8
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
Streptavidin
27
21
21
16
16
16
10
10
10
10
10
10
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
Application
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
245
145
97
73
71
59
53
47
33
30
28
6
4
4
3
2
2
2
1
1
1
Options
Immunogen Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Clone 60B1220-1
Isotype IgG1 kappa
Specificity Detects ~180 kDa. It will cross-react with mouse DNMT1.
Purification Protein G Purified
Antigen
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Gene ID 1786
NCBI Accession NP_001370
UniProt P26358
Pathways SARS-CoV-2 Protein Interactome
Application Notes
  • WB (1:1000)
  • IHC (1:1000)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of ABIN2486011 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Image no. 1 for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody (Streptavidin) (ABIN2486011) Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B12...
Image no. 2 for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody (Streptavidin) (ABIN2486011) Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein u...
Image no. 3 for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody (Streptavidin) (ABIN2486011) Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B12...