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HSPB2 antibody (Biotin)

HSPB2 Reactivity: Human WB, IF, ICC Host: Rabbit Polyclonal Biotin
Catalog No. ABIN2486040
  • Target See all HSPB2 Antibodies
    HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))
    Reactivity
    • 43
    • 27
    • 27
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 58
    • 2
    Rabbit
    Clonality
    • 58
    • 2
    Polyclonal
    Conjugate
    • 26
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HSPB2 antibody is conjugated to Biotin
    Application
    • 47
    • 19
    • 18
    • 14
    • 13
    • 13
    • 13
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~27 kDa.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein A Purified
    Immunogen
    Synthetic peptide
    Top Product
    Discover our top product HSPB2 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2486040 was sufficient for detection of MKBP in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.02 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))
    Alternative Name
    HSPB2 (HSPB2 Products)
    Synonyms
    zgc:112532 antibody, HSP27 antibody, Hs.78846 antibody, LOH11CR1K antibody, MKBP antibody, 27kDa antibody, 2810021G24Rik antibody, heat shock protein family B (small) member 2 antibody, heat shock protein, alpha-crystallin-related, b2 antibody, heat shock protein 2 antibody, HSPB2 antibody, hspb2 antibody, Hspb2 antibody
    Background
    HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30 % sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSPs, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP's localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).
    Gene ID
    3316
    NCBI Accession
    NP_001532
    UniProt
    Q16082
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