Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SCNN1A antibody (AA 629-650) (Atto 488)

SCNN1A Reactivity: Rat WB, IHC, IF, ICC Host: Rabbit Polyclonal Atto 488
Catalog No. ABIN2486418
  • Target See all SCNN1A Antibodies
    SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))
    Binding Specificity
    • 63
    • 22
    • 21
    • 10
    • 10
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 629-650
    Reactivity
    • 99
    • 45
    • 39
    • 20
    • 10
    Rat
    Host
    • 82
    • 43
    Rabbit
    Clonality
    • 82
    • 43
    Polyclonal
    Conjugate
    • 28
    • 11
    • 10
    • 10
    • 10
    • 10
    • 9
    • 9
    • 9
    • 8
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SCNN1A antibody is conjugated to Atto 488
    Application
    • 115
    • 103
    • 36
    • 35
    • 34
    • 21
    • 10
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~83 kDa.
    Cross-Reactivity
    Hamster, Human, Mouse, Rat, Xenopus laevis
    Purification
    Protein A Purified
    Immunogen
    Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
    Top Product
    Discover our top product SCNN1A Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2486418 was sufficient for detection of gamma-ENaC in 20 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))
    Alternative Name
    ENaC (SCNN1A Products)
    Synonyms
    BESC2 antibody, ENaCa antibody, ENaCalpha antibody, SCNEA antibody, SCNN1 antibody, ENaC antibody, Scnn1 antibody, mENaC antibody, SCNN1A antibody, alphaxENaC antibody, besc2 antibody, enaca antibody, scnn1 antibody, ENAC antibody, sodium channel epithelial 1 alpha subunit antibody, sodium channel, nonvoltage-gated 1 alpha antibody, sodium channel, non voltage gated 1 alpha subunit L homeolog antibody, Scnn1a antibody, SCNN1A antibody, scnn1a.L antibody
    Background
    The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
    Gene ID
    24768
    NCBI Accession
    NP_058742
    UniProt
    P37091
You are here:
Support