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GRP78 antibody (Atto 390)

HSPA5 Reactivity: Rat WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal Atto 390
Catalog No. ABIN2486748
  • Target See all GRP78 (HSPA5) Antibodies
    GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
    Reactivity
    • 186
    • 123
    • 106
    • 39
    • 38
    • 36
    • 34
    • 32
    • 31
    • 20
    • 15
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 131
    • 90
    • 9
    • 3
    • 1
    • 1
    Rabbit
    Clonality
    • 134
    • 102
    Polyclonal
    Conjugate
    • 100
    • 21
    • 17
    • 14
    • 13
    • 12
    • 9
    • 8
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GRP78 antibody is conjugated to Atto 390
    Application
    • 221
    • 104
    • 88
    • 85
    • 77
    • 34
    • 31
    • 28
    • 26
    • 21
    • 13
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificity
    Detects ~78 kDa.
    Cross-Reactivity
    Cow, Dog, Fusarium, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogen
    Rat GRP78 (Bip) synthetic peptide conjugated to KLH
    Top Product
    Discover our top product HSPA5 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN2486748 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
    Alternative Name
    GRP78 (HSPA5 Products)
    Synonyms
    GRP78 antibody, BiP antibody, GRP-78 antibody, grp78 antibody, hspa5a antibody, BIP antibody, MIF2 antibody, AL022860 antibody, AU019543 antibody, Bip antibody, D2Wsu141e antibody, D2Wsu17e antibody, Grp78 antibody, Hsce70 antibody, SEZ-7 antibody, Sez7 antibody, baffled antibody, mBiP antibody, cb865 antibody, fb60h09 antibody, fi36d04 antibody, wu:fb60h09 antibody, wu:fi36d04 antibody, zgc:55994 antibody, zgc:77606 antibody, 78 kDa glucose-regulated protein antibody, heat shock protein family A (Hsp70) member 5 antibody, BiP/GRP78 antibody, glucose-regulated protein 78 antibody, putative glucose-regulated protein 78 antibody, Hsp70 family ATPase KAR2 antibody, heat shock protein family A (Hsp70) member 5 S homeolog antibody, heat shock 70 kDa protein 5a antibody, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, heat shock protein 5 antibody, heat shock protein family A member 5 antibody, CpipJ_CPIJ003550 antibody, HSPA5 antibody, grp78 antibody, LOC100533358 antibody, BiP/grp78 antibody, Tc00.1047053506585.40 antibody, Tb11.02.5450 antibody, Tb11.02.5500 antibody, LMJF_28_1200 antibody, KAR2 antibody, LOC100135840 antibody, hspa5.S antibody, hspa5 antibody, Hspa5 antibody
    Background
    GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
    Gene ID
    25617
    NCBI Accession
    NP_037215
    UniProt
    P06761
    Pathways
    Thyroid Hormone Synthesis, ER-Nucleus Signaling
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