Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

AKT2 antibody (C-Term) (Atto 488)

AKT2 Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal Atto 488
Catalog No. ABIN2486851
  • Target See all AKT2 Antibodies
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Binding Specificity
    • 23
    • 22
    • 17
    • 16
    • 15
    • 15
    • 13
    • 9
    • 7
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 192
    • 105
    • 68
    • 16
    • 15
    • 13
    • 13
    • 13
    • 11
    • 10
    • 10
    • 10
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    Human
    Host
    • 170
    • 29
    • 5
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 171
    • 35
    Polyclonal
    Conjugate
    • 97
    • 17
    • 16
    • 11
    • 9
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This AKT2 antibody is conjugated to Atto 488
    Application
    • 176
    • 114
    • 77
    • 37
    • 28
    • 27
    • 26
    • 20
    • 19
    • 10
    • 9
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
    Cross-Reactivity
    Chicken, Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogen
    A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:25)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:3000 dilution of ABIN2486851 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    TBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Alternative Name
    AKT2 (AKT2 Products)
    Synonyms
    AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody
    Background
    Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
    Gene ID
    208
    NCBI Accession
    NP_001617
    UniProt
    P31751
    Pathways
    PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
You are here:
Support