AKT2 antibody (C-Term) (Atto 488)
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- Target See all AKT2 Antibodies
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AKT2 antibody is conjugated to Atto 488
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
- Cross-Reactivity
- Chicken, Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis
- Purification
- Peptide Affinity Purified
- Immunogen
- A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:25)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:3000 dilution of ABIN2486851 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- TBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Alternative Name
- AKT2 (AKT2 Products)
- Synonyms
- AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody
- Background
- Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
- Gene ID
- 208
- NCBI Accession
- NP_001617
- UniProt
- P31751
- Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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