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5-Hydroxymethylcytosine (5-hmC) antibody Primary Antibody

5-hmC DB, hmeDIP, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2486985
$245.00
Plus shipping costs $45.00
50 μL
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  • Target
    5-Hydroxymethylcytosine (5-hmC)
    Reactivity
    Please inquire
    Host
    • 14
    • 3
    Rabbit
    Clonality
    • 11
    • 6
    Polyclonal
    Conjugate
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), ELISA
    Purpose
    Polyclonal Antibody to detect 5-hmC in human samples.
    Characteristics
    Polyclonal Antibody to detect 5-hmC in human samples.
    Purification
    Whole Anti-Sera
    Immunogen
    Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
    Isotype
    IgG
  • Application Notes
    hMeDIP: 2.5 μg/IP, Dot Blot: 1:200, ELISA: 1:500.
    Comment

    Suggested positive control:
    ELISA: Peptide, hmEdIP, Dot blot: Peptides.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In PBS with 0.05 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    The antibody solution should be gently mixed before use.
    Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    5-Hydroxymethylcytosine (5-hmC)
    Alternative Name
    5-HmC
    Target Type
    Chemical
    Background
    5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0.6 % of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is abundant in brain tissue, especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique rabbit polyclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.
    Synonyms: 5-hydroxymethylcytosine
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