COL2A1 antibody (Chain alpha 1)

Catalog No. ABIN113964

Product Details anti-COL2A1 Antibody

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Binding Specificity: Chain alpha 1

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Specificity: This antibody is specific for Rat Collagen type II (100 %). Cross Reactions: Rat Collagen I, III and V: < 0.1 % Rat Elastin, Rat-Fibronectin: < 0.1 % (solid phase RIA at 1/100 dilution).

Purification: Ion Exchange Chromatography

Immunogen: Purified collagen type II from fetal rat cartilage

Application Details

Application Notes: IF: 1: 40. Immunohistochemistry on frozen and paraffin sections: (P) 1: 100 - 1: 500, 60 min at RT or2-8C over night. ELISA. RIA. Positive control: rat cartilage. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.

Concentration: >/= 1.0 mg/mL

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Abstract: COL2A1 Products

Synonyms: ANFH antibody, AOM antibody, COL11A3 antibody, SEDC antibody, Col2 antibody, Col2a antibody, Col2a-1 antibody, Del1 antibody, Dmm antibody, Lpk antibody, M100856 antibody, Rgsc856 antibody, CG2A1A antibody, COLLII antibody, col2a1a antibody, CB11 antibody, col2a1 antibody, coll2a1 antibody, fb38c06 antibody, fc10c01 antibody, wu:fb38c06 antibody, wu:fc10c01 antibody, collagen type II alpha 1 chain antibody, collagen, type II, alpha 1 antibody, collagen, type II, alpha 1 L homeolog antibody, collagen, type II, alpha 1 S homeolog antibody, collagen, type II, alpha 1a antibody, COL2A1 antibody, Col2a1 antibody, col2a1 antibody, col2a1.L antibody, col2a1.S antibody, col2a1a antibody

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type II Collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.Synonyms: Alpha-1 type II collagen, COL2A1

Gene ID: 25412

NCBI Accession: NP_037061

UniProt: P05539

Pathways: Sensory Perception of Sound, Growth Factor Binding