PINK1 antibody (PTEN Induced Putative Kinase 1) (AA 175-250)

Details for Product anti-PINK1 Antibody No. ABIN249446, Supplier: Log in to see
Antigen
  • BEST:GH23468
  • CG4523
  • Dmel\\CG4523
  • PINK
  • PINK1
  • dPINK1
  • dPink1
  • pink1
  • wu:fc39e12
  • zgc:101729
  • BRPK
  • PARK6
  • 1190006F07Rik
  • AU042772
  • AW557854
  • mFLJ00387
  • PTEN-induced putative kinase 1
  • PTEN induced putative kinase 1
  • Pink1
  • PINK1
  • pink1
Epitope
AA 175-250
57
51
39
32
19
18
18
9
9
9
7
7
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
247
180
110
4
2
2
1
1
Host
Rabbit
191
84
17
2
Clonality
Polyclonal
Conjugate
This PINK1 antibody is un-conjugated
16
15
13
9
9
9
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
254
127
118
63
57
57
22
8
3
3
2
1
Options
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Supplier Product No.
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Immunogen A synthetic peptide made to the human PINK1 protein sequence (between residues 175-250). [Swiss-Prot Q9BXM7]
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others PINK1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PINK1 (PINK1 Antibody Abstract)
Background Gene Symbol: PINK1
Molecular Weight Theoretical MW: 63 kDa
Gene ID 65018
UniProt Q9BXM7
Application Notes Western Blot 1:500-1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation, Immunohistochemistry-Paraffin, Peptide ELISA 1:100-1:2000This PINK1 antibody can be used for ICC, Peptide ELISA and Western blot, where specific bands are seen at 48, 55 and 63 kDa. In WB, this antibody has been used in valinomycin and CCCP treated HeLa whole cell lysate. Use in IP reported in scientific literature (PMID 22078885) Use in paraffin-sections reported in scientific literature (PMID 25083992). In Western blot it is highly recommended to use 1-5 % w/v BSA in TBS with 0.1 % Tween-20 for all incubations.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - Immunocytochemis...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Whole cell protein from HeLa cells treated...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Western blot image of PINK1 antibody (BC10...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in HeLa whole cell lysat...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in mouse liver and hypat...
Immunofluorescence (IF) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - HeLa cells were ...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody - WB analysis of lysates derived from hTERT-RPE1 cells ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Immunohistochemistry-Paraffin: PINK1 Antibody - Rabbit heart tissue. Image from veri...
Product cited in: Kim, Ham, Jo, Lee, Lee, Shin, Lee: "CRISPR-Cas9 Mediated Telomere Removal Leads to Mitochondrial Stress and Protein Aggregation." in: International journal of molecular sciences, Vol. 18, Issue 10, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Yoo, Chung: "The ubiquitin E3 ligase CHIP promotes proteasomal degradation of the serine/threonine protein kinase PINK1 during staurosporine-induced cell death." in: The Journal of biological chemistry, Vol. 293, Issue 4, pp. 1286-1297, 2018 (PubMed). (Sample species: Human).

Fiesel, James, Hudec, Springer: "Mitochondrial targeted HSP90 inhibitor Gamitrinib-TPP (G-TPP) induces PINK1/Parkin-dependent mitophagy." in: Oncotarget, Vol. 8, Issue 63, pp. 106233-106248, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kumar, Shaha: "SESN2 facilitates mitophagy by helping Parkin translocation through ULK1 mediated Beclin1 phosphorylation." in: Scientific reports, Vol. 8, Issue 1, pp. 615, 2018 (PubMed).

Goiran, Duplan, Rouland, El Manaa, Lauritzen, Dunys, You, Checler, Alves da Costa: "Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation." in: Cell death and differentiation, Vol. 25, Issue 5, pp. 873-884, 2018 (PubMed).

Bueno, Brands, Voltz, Fiedler, Mays, St Croix, Sembrat, Mallampalli, Rojas, Mora: "ATF3 represses PINK1 gene transcription in lung epithelial cells to control mitochondrial homeostasis." in: Aging cell, Vol. 17, Issue 2, 2018 (PubMed).

Biel, Rao: "Mitochondrial dysfunction activates lysosomal-dependent mitophagy selectively in cancer cells." in: Oncotarget, Vol. 9, Issue 1, pp. 995-1011, 2018 (PubMed).

Hawk, Gorsuch, Fagan, Lee, Kim, Hamann, Mason, Weigel, Tsegaye, Shen, Shuff, Zuo, Hu, Jiang, Chapman, Leevy, DeBerardinis, Overholtzer, Schafer: "RIPK1-mediated induction of mitophagy compromises the viability of extracellular-matrix-detached cells." in: Nature cell biology, Vol. 20, Issue 3, pp. 272-284, 2018 (PubMed).

Gu, Qi, Feng, Ma, Gao, Zhang: "Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway." in: Toxicology letters, Vol. 291, pp. 92-100, 2018 (PubMed).

Sun, Yao, Zhang, Zhu, Liu, Ci, Xie, Carlson, Rothermel, Sun, Levine, Hill, Wolf, Minei, Zang: "Beclin-1-Dependent Autophagy Protects the Heart During Sepsis." in: Circulation, 2018 (PubMed).

Marcassa, Kallinos, Jardine, Rusilowicz-Jones, Martinez, Kuehl, Islinger, Clague, Urbé: "Dual role of USP30 in controlling basal pexophagy and mitophagy." in: EMBO reports, Vol. 19, Issue 7, 2018 (PubMed).

Thomas, Zhang, Stefely, Veiga, Thomas, Kozma, Mercer: "Mitochondrial Complex I Activity Is Required for Maximal Autophagy." in: Cell reports, Vol. 24, Issue 9, pp. 2404-2417.e8, 2018 (PubMed).

Li, Fu, Shen, Zhou, Hu, Liu, Li, Zhang, Liu, Zhang, Huang, Zhang, Gao: "Polyphyllin I induces mitophagic and apoptotic cell death in human breast cancer cells by increasing mitochondrial PINK1 levels." in: Oncotarget, Vol. 8, Issue 6, pp. 10359-10374, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sato, Furuya: "Induction of PINK1/Parkin-Mediated Mitophagy." in: Methods in molecular biology (Clifton, N.J.), 2017 (PubMed).

Martinez, Lectez, Ramirez, Popp, Sutherland, Urbé, Dittmar, Clague, Mayor: "Quantitative proteomic analysis of Parkin substrates in Drosophila neurons." in: Molecular neurodegeneration, Vol. 12, Issue 1, pp. 29, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Fruit Fly (Drosophila melanogaster)).

Orr, Rutaganira, de Roulet, Huang, Hertz, Shokat, Nakamura: "Long-term oral kinetin does not protect against α-synuclein-induced neurodegeneration in rodent models of Parkinson's disease." in: Neurochemistry international, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Zhang, Liu, Bunker, Ramirez, Lee, Peng, Tan, Eckhardt, Chapnick, Liu: "Sorafenib targets the mitochondrial electron transport chain complexes and ATP synthase to activate the PINK1-Parkin pathway and modulate cellular drug response." in: The Journal of biological chemistry, Vol. 292, Issue 36, pp. 15105-15120, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Xiao, Goh, Xiao, Xian, Lim, Liou: "Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin." in: The Journal of biological chemistry, Vol. 292, Issue 40, pp. 16697-16708, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Kravic, Harbauer, Romanello, Simeone, Vögtle, Kaiser, Straubinger, Huraskin, Böttcher, Cerqua, Martin, Poveda-Huertes, Buttgereit, Rabalski, Heuss, Rudolf, Friedrich, Litchfield, Marber, Salviati et al.: "In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy. ..." in: Autophagy, pp. 1-25, 2017 (PubMed). (Sample species: Mouse (Murine)).

Tang, Han, Yan, Zhu, Liu, Liu, He, Tan, Liu, Liu, Sun, Duan, Peng, Liu, Yin, Zhang, Dong: "PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against renal ischemia-reperfusion injury." in: Autophagy, pp. 1-18, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

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