PINK1 antibody (PTEN Induced Putative Kinase 1) (AA 175-250)

Details for Product anti-PINK1 Antibody No. ABIN249446, Supplier: Log in to see
Antigen
  • BEST:GH23468
  • CG4523
  • Dmel\\CG4523
  • PINK
  • PINK1
  • dPINK1
  • dPink1
  • pink1
  • wu:fc39e12
  • zgc:101729
  • BRPK
  • PARK6
  • 1190006F07Rik
  • AU042772
  • AW557854
  • mFLJ00387
  • PTEN-induced putative kinase 1
  • PTEN induced putative kinase 1
  • Pink1
  • PINK1
  • pink1
Epitope
AA 175-250
50
39
33
26
17
14
13
9
9
9
7
7
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
213
166
104
4
2
2
1
1
1
1
Host
Rabbit
169
68
16
2
Clonality
Polyclonal
Conjugate
This PINK1 antibody is un-conjugated
14
13
10
8
8
8
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
220
103
94
58
52
50
22
6
3
3
2
1
Options
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide made to the human PINK1 protein sequence (between residues 175-250). [Swiss-Prot Q9BXM7]
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others PINK1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PINK1 (PINK1 Antibody Abstract)
Background Gene Symbol: PINK1
Molecular Weight Theoretical MW: 63 kDa
Gene ID 65018
UniProt Q9BXM7
Application Notes Western Blot 1:500-1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation, Immunohistochemistry-Paraffin, Peptide ELISA 1:100-1:2000This PINK1 antibody can be used for ICC, Peptide ELISA and Western blot, where specific bands are seen at 48, 55 and 63 kDa. In WB, this antibody has been used in valinomycin and CCCP treated HeLa whole cell lysate. Use in IP reported in scientific literature (PMID 22078885) Use in paraffin-sections reported in scientific literature (PMID 25083992). In Western blot it is highly recommended to use 1-5 % w/v BSA in TBS with 0.1 % Tween-20 for all incubations.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - Immunocytochemis...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Whole cell protein from HeLa cells treated...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Western blot image of PINK1 antibody (BC10...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in HeLa whole cell lysat...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in mouse liver and hypat...
Immunofluorescence (IF) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - HeLa cells were ...
Western Blotting (WB) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Western Blot: PINK1 Antibody - WB analysis of lysates derived from hTERT-RPE1 cells ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 175-250) antibody (ABIN249446) Immunohistochemistry-Paraffin: PINK1 Antibody - Rabbit heart tissue. Image from veri...
Product cited in: Li, Fu, Shen, Zhou, Hu, Liu, Li, Zhang, Liu, Zhang, Huang, Zhang, Gao: "Polyphyllin I induces mitophagic and apoptotic cell death in human breast cancer cells by increasing mitochondrial PINK1 levels." in: Oncotarget, Vol. 8, Issue 6, pp. 10359-10374, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sato, Furuya: "Induction of PINK1/Parkin-Mediated Mitophagy." in: Methods in molecular biology (Clifton, N.J.), 2017 (PubMed).

Martinez, Lectez, Ramirez, Popp, Sutherland, Urbé, Dittmar, Clague, Mayor: "Quantitative proteomic analysis of Parkin substrates in Drosophila neurons." in: Molecular neurodegeneration, Vol. 12, Issue 1, pp. 29, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Fruit Fly (Drosophila melanogaster)).

Orr, Rutaganira, de Roulet, Huang, Hertz, Shokat, Nakamura: "Long-term oral kinetin does not protect against α-synuclein-induced neurodegeneration in rodent models of Parkinson's disease." in: Neurochemistry international, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Zhang, Liu, Bunker, Ramirez, Lee, Peng, Tan, Eckhardt, Chapnick, Liu: "Sorafenib targets the mitochondrial electron transport chain complexes and ATP synthase to activate the PINK1-Parkin pathway and modulate cellular drug response." in: The Journal of biological chemistry, Vol. 292, Issue 36, pp. 15105-15120, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Xiao, Goh, Xiao, Xian, Lim, Liou: "Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin." in: The Journal of biological chemistry, Vol. 292, Issue 40, pp. 16697-16708, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Ivankovic, Chau, Schapira, Gegg: "Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy." in: Journal of neurochemistry, Vol. 136, Issue 2, pp. 388-402, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Martín-Maestro, Gargini, Perry, Avila, García-Escudero: "PARK2 enhancement is able to compensate mitophagy alterations found in sporadic Alzheimer's disease." in: Human molecular genetics, Vol. 25, Issue 4, pp. 792-806, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Scott, Wicker, Suganya, Dhar, Pittman, Horbinski, Izumi: "Polyubiquitination of apurinic/apyrimidinic endonuclease 1 by Parkin." in: Molecular carcinogenesis, 2016 (PubMed).

Akabane, Matsuzaki, Yamashita, Arai, Okatsu, Kanki, Matsuda, Oka: "Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy." in: The Journal of biological chemistry, Vol. 291, Issue 31, pp. 16162-74, 2016 (PubMed). (Sample species: Human).

Kim, Fiesel, Belmonte, Hudec, Wang, Kim, Nelson, Springer, Kim: "miR-27a and miR-27b regulate autophagic clearance of damaged mitochondria by targeting PTEN-induced putative kinase 1 (PINK1)." in: Molecular neurodegeneration, Vol. 11, Issue 1, pp. 55, 2016 (PubMed). Further details: Western Blotting

Xu, Zhi, Shao, Wang, Yang, Xia: "Cytoprotection against Hypoxic and/or MPP⁺ Injury: Effect of δ-Opioid Receptor Activation on Caspase 3." in: International journal of molecular sciences, Vol. 17, Issue 8, 2016 (PubMed). Further details: Western Blotting

Zhang, Xue, Li, Tang, Wan, Wang, Tan, Tan, Han, Tian, Billiar, Tao, Zhang: "BNIP3 Protein Suppresses PINK1 Kinase Proteolytic Cleavage to Promote Mitophagy." in: The Journal of biological chemistry, Vol. 291, Issue 41, pp. 21616-21629, 2016 (PubMed). Further details: Immunocytochemistry,Immunoprecipitation,Immunofluorescence

Wai, Saita, Nolte, Müller, König, Richter-Dennerlein, Sprenger, Madrenas, Mühlmeister, Brandt, Krüger, Langer: "The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L." in: EMBO reports, Vol. 17, Issue 12, pp. 1844-1856, 2016 (PubMed). Method employed by authors: Immunofluorescence (IF)

Wettengel, Reautschnig, Geisler, Kahle, Stafforst: "Harnessing human ADAR2 for RNA repair - Recoding a PINK1 mutation rescues mitophagy." in: Nucleic acids research, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Berezhnov, Soutar, Fedotova, Frolova, Plun-Favreau, Zinchenko, Abramov: "Intracellular pH Modulates Autophagy and Mitophagy." in: The Journal of biological chemistry, Vol. 291, Issue 16, pp. 8701-8, 2016 (PubMed).

Jamil, Hojabrpour, Duronio: "The small molecule 2-phenylethynesulfonamide induces covalent modification of p53." in: Biochemical and biophysical research communications, Vol. 482, Issue 1, pp. 154-158, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Callegari, Oeljeklaus, Warscheid, Dennerlein, Thumm, Rehling, Dudek: "Phospho-ubiquitin-PARK2 complex as a marker for mitophagy defects." in: Autophagy, Vol. 13, Issue 1, pp. 201-211, 2016 (PubMed). (Sample species: Human).

Mannam, Rauniyar, Lam, Luo, Lee, Srivastava: "MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation." in: Free radical biology & medicine, Vol. 101, pp. 102-115, 2016 (PubMed).

Choi, Choi, Kang, Lim, Park, Lee, Park, Moon, Kim, Choi, Joe, Choi, Yoon: "PINK1 positively regulates HDAC3 to suppress dopaminergic neuronal cell death." in: Human molecular genetics, Vol. 24, Issue 4, pp. 1127-41, 2015 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

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