GIPC1 antibody (GIPC PDZ Domain Containing Family, Member 1) (C-Term)

Details for Product anti-GIPC1 Antibody No. ABIN250102, Supplier: Log in to see
Antigen
  • GAMMA-TIP2
  • SALT-STRESS INDUCIBLE PROTEIN
  • SALT-STRESS INDUCIBLE TONOPLAST INTRINSIC PROTEIN
  • SITIP
  • TIP1;2
  • TONOPLAST INTRINSIC PROTEIN 2
  • tonoplast intrinsic protein 2
  • GIPC
  • XGIPC
  • gipc3
  • iip-1
  • Kermit
  • semcap
  • glut1cbp
  • rgs19ip1
  • synectiin
  • GIPC1
  • TIP-2
  • kermit
  • C19orf3
  • GLUT1CBP
  • Hs.6454
  • IIP-1
  • NIP
  • RGS19IP1
  • SEMCAP
  • SYNECTIIN
  • SYNECTIN
  • Glut1CIP
  • Rgs19ip1
  • Semcap1
  • TaxIP2
  • Gipc
  • Rgs19
  • tonoplast intrinsic protein 2
  • GIPC PDZ domain containing family member 1
  • RGS-GAIP interacting protein GIPC
  • PDZ domain-containing protein GIPC3
  • putative rgs-gaip interacting protein gipc
  • GIPC PDZ domain containing family, member 1
  • GIPC PDZ domain containing family member 1 L homeolog
  • TIP2
  • gipc1
  • GIPC1
  • LOC692815
  • LOC5576629
  • Smp_170870
  • gipc1.L
  • Gipc1
Alternatives
anti-Human GIPC1 antibody for ELISA
Epitope
C-Term
17
14
13
6
4
3
3
3
2
1
1
1
1
1
1
Reactivity
Human
92
47
34
6
4
4
2
2
2
1
1
1
1
Host
Goat
66
15
10
2
Clonality
Polyclonal
Conjugate
This GIPC1 antibody is un-conjugated
5
5
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
74
43
20
13
10
4
3
3
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Peptide with sequence C-GAIGDAKVGRY corresponding to C-Terminus according to NP_974197.1, NP_974199.1, NP_974196.1, NP_974198.1, NP_974223.1.
Specificity This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others GIPC1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name GIPC1 (GIPC1 Antibody Abstract)
Background Gene Symbol: GIPC1
Molecular Weight Theoretical MW: 36 kDa
Gene ID 10755
UniProt O14908
Pathways Dicarboxylic Acid Transport
Application Notes Western Blot 0.03 - 0.1 μg/mL, Immunohistochemistry 1:10 - 1:500, Immunohistochemistry-Paraffin 10 μg/mL, Peptide ELISA Detection limit 1:128000WB: Approx. 38 kDa band and strong 23-24 kDa band bands observed in lysates of HeLa, and approx. 24 kDa band also observed in human pancreas, kidney and muscle lysates (calculated MW of 36.0 kDa band according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1 kDa band according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GIPC PDZ Domain Containing Family, Member 1 (GIPC1) (C-Term) antibody (ABIN250102) ABIN250102 staining (1µg/ml) of Hela lysate (RIPA buffer, 30µg total protein per lane...
Immunohistochemistry (IHC) image for anti-GIPC PDZ Domain Containing Family, Member 1 (GIPC1) (C-Term) antibody (ABIN250102) ABIN250102 (10?g/ml) staining of paraffin embedded Human Kidney. Microwaved antigen r...
Western Blotting (WB) image for anti-GIPC PDZ Domain Containing Family, Member 1 (GIPC1) (C-Term) antibody (ABIN250102) Western Blot: GIPC1 Antibody [ABIN250102] - GIPC1 in HeLa cell lysate (RIPA buffer, 3...
Western Blotting (WB) image for anti-GIPC PDZ Domain Containing Family, Member 1 (GIPC1) (C-Term) antibody (ABIN250102) Western Blot: GIPC1 Antibody [ABIN250102] - HEK293 overexpressing GIPC1 isoform 1 (RC...
Product cited in: Wu, Haruta, Wei: "GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion." in: The Journal of biological chemistry, Vol. 285, Issue 37, pp. 28643-50, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

De Vries, Lou, Zhao, Zheng, Farquhar: "GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12340-5, 1998 (PubMed).

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