Spartan antibody (C-Term)

Catalog No. ABIN2505865

Product Details anti-Spartan Antibody

Target: Spartan (C1orf124) (Chromosome 1 Open Reading Frame 124 (C1orf124))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spartan antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QIKSSGNDPK YSTTTAQNSS SSSSQSKMVN CPVCQNEVLE SQINEHLDWC

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against SPRTN. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human SPRTN

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Spartan

Target: Spartan (C1orf124) (Chromosome 1 Open Reading Frame 124 (C1orf124))

Alternative Name: SPRTN (C1orf124 Products)

Synonyms: Spartan antibody, c1orf124 antibody, C1orf124 antibody, DDDL1880 antibody, DVC1 antibody, PRO4323 antibody, dJ876B10.3 antibody, C28H1orf124 antibody, SprT-like N-terminal domain antibody, sprtn antibody, SPRTN antibody

Background: SPRTN is a regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. SPRTN is recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. It facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. It is also required for localization of DNA polymerase eta (POLH) to sites of UV damage.
Alias Symbols: C1orf124, DDDL1880, PRO4323, Spartan, dJ876B10.3
Protein Interaction Partner: H2BFS, UBR5, FAM120A, STRAP, CKAP4, MAGED2, HIST2H2BF, HIST1H2BK, POLD4, ZC3HAV1, POLD3, DDX17, SYNCRIP, TRIM28, LRPPRC, HUWE1, HNRNPDL, AKAP12, RBM39, H1FX, HIST1H2BH, HIST1H2BM, HIST1H2BN, HIST1H2BL, HIST1H2BG, VIM, VDAC1, UBB, TUFM, DNAJC7, TMPO, STAU1
Protein Size: 489

Molecular Weight: 55 kDa

Gene ID: 83932

NCBI Accession: NM_032018, NP_114407

UniProt: Q9H040