Aryl Hydrocarbon Receptor antibody (AHR) (C-Term)

Details for Product anti-AHR Antibody No. ABIN250676, Supplier: Log in to see
Antigen
  • AHR
  • ahr1
  • ahr
  • bHLHe76
  • Ah
  • Ahh
  • Ahre
  • In
  • aryl hydrocarbon receptor S homeolog
  • aryl hydrocarbon receptor
  • aryl-hydrocarbon receptor
  • ahr.S
  • AHR
  • ahr
  • Ahr
Alternatives
anti-Human Aryl Hydrocarbon Receptor antibody for Chromatin Immunoprecipitation
Epitope
C-Term
30
24
21
16
15
10
9
8
7
6
5
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
233
108
87
16
8
5
4
4
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
173
45
29
8
Clonality
Polyclonal
Conjugate
This Aryl Hydrocarbon Receptor antibody is un-conjugated
8
7
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
173
127
123
73
65
44
30
21
13
8
5
5
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others Aryl Hydrocarbon Receptor products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name AHR (AHR Antibody Abstract)
Background Gene Symbol: AHR
Molecular Weight Theoretical MW: 96 kDa
Gene ID 196
UniProt P35869
Pathways Regulation of Cell Size
Application Notes Western Blot 1:500-1:2000, Simple Western 1:200, ELISA 1:100-1:2000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:500-1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This Aryl hydrocarbon Receptor antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot. In Western blot a band is seen approx. 90 to 105 kDa representing AHR (molecular weight varies by species and by strain). In ICC/IF, cytoplasmic staining was observed in MCF-7 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for Aryl hydrocarbon Receptor antibody
Western Blot Protocol:
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.ICC/IF Protocol for Aryl hydrocarbon Receptor antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Aryl Hydrocarbon Receptor (AHR) (C-Term) antibody (ABIN250676) Detection of AhR in mouse liver cytosol using ABIN250676.
Simple Western (SimWes) image for anti-Aryl Hydrocarbon Receptor (AHR) (C-Term) antibody (ABIN250676) Simple Western: AHR Antibody [ABIN250676] - Simple Western lane view shows a specific...
Immunohistochemistry (IHC) image for anti-Aryl Hydrocarbon Receptor (AHR) (C-Term) antibody (ABIN250676) Immunohistochemistry: AHR Antibody [ABIN250676] - Staining of Aryl hydrocarbon Recept...
Immunofluorescence (IF) image for anti-Aryl Hydrocarbon Receptor (AHR) (C-Term) antibody (ABIN250676) Immunocytochemistry/Immunofluorescence: AHR Antibody [ABIN250676] - Aryl hydrocarbon ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Aryl Hydrocarbon Receptor (AHR) (C-Term) antibody (ABIN250676) Immunocytochemistry/Immunofluorescence: AHR Antibody - HeLa cells were fixed for 10 ...
Product cited in: Bradley, Cryar, El Hajj, El Hajj, Gardner: "Exposure to diesel exhaust particulates induces cardiac dysfunction and remodeling." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 115, Issue 7, pp. 1099-106, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Did you look for something else?