TUBB antibody (Tubulin, beta)

Details for Product anti-TUBB Antibody No. ABIN252426
Target
Reactivity
Cat, Chicken, Cow, Dog, Human, Monkey, Mouse, Primate, Rat, Xenopus laevis
204
157
146
28
26
23
21
18
16
16
16
11
9
7
7
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
116
110
1
1
1
1
Clonality
Polyclonal
Conjugate
This TUBB antibody is un-conjugated
10
10
10
8
7
7
7
7
3
3
3
3
3
3
2
2
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
156
53
51
50
40
37
25
13
10
9
6
5
3
2
2
1
1
1
1
Options
Immunogen A synthetic peptide corresponding to a portion of amino acids 400-450 of human b-tubulin was used as immunogen for this antibody, GenBank no. gi 27227551 gb AAN85571.1 .
Specificity The amino acid sequence used as immunogen has 100 % identity in beta I and beta II tubulin.
Purification Protein G purified
Target
Alternative Name beta Tubulin (TUBB Antibody Abstract)
Background Gene Symbol: TUBB
Gene ID 203068
UniProt P07437
Pathways Microtubule Dynamics, M Phase
Application Notes Western Blot 0.1-1 μg/mL, Simple Western 1:50, Immunohistochemistry 1:100-1:1000, Immunohistochemistry-Paraffin 5 - 10 μg/mLIn Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Images
Image no. 1 for anti-Tubulin, beta (TUBB) antibody (ABIN252426) Western blot analysis of beta tubulin in A: human brain (40141), B: mouse brain (4012...
Image no. 2 for anti-Tubulin, beta (TUBB) antibody (ABIN252426) Immunohistochemical analysis of b-tubulin in paraffin-embedded formalin-fixed human b...
Image no. 3 for anti-Tubulin, beta (TUBB) antibody (ABIN252426) Simple Western: beta Tubulin Antibody [ABIN2524269] - Simple Western lane view shows ...
Image no. 4 for anti-Tubulin, beta (TUBB) antibody (ABIN252426) Immunohistochemistry-Paraffin: beta Tubulin Antibody [ABIN2524269] - Formalin-fixed p...
Product cited in: Ringuette Goulet, Bernard, Tremblay, Chabaud, Bolduc, Pouliot: "Exosomes Induce Fibroblast Differentiation into Cancer-Associated Fibroblasts through TGFβ Signaling." in: Molecular cancer research : MCR, Vol. 16, Issue 7, pp. 1196-1204, 2018 (PubMed).

Chen, Arumugam, Leanage, Tieng, Yadav, Ullmann, She, Truong, Ruitenberg, Reutens: "Disease-modifying effect of intravenous immunoglobulin in an experimental model of epilepsy." in: Scientific reports, Vol. 7, pp. 40528, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Williams, Balsor, Beshara, Beston, Jones, Murphy: "Experience-dependent central vision deficits: Neurobiology and visual acuity." in: Vision research, 2015 (PubMed). (Sample species: Cat (Feline)). Further details: Western Blotting

Murphy, Balsor, Beshara, Siu, Pinto: "A high-throughput semi-automated preparation for filtered synaptoneurosomes." in: Journal of neuroscience methods, Vol. 235, pp. 35-40, 2014 (PubMed). Further details: Western Blotting

Saha: "LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy." in: Autophagy, Vol. 8, Issue 11, pp. 1643-56, 2013 (PubMed).

Li, Guan, Yang: "Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes." in: Regulatory peptides, Vol. 178, Issue 1-3, pp. 16-20, 2012 (PubMed).

Kamal, Sreekanth, Kumar, Shankaraiah, Balakishan, Ramaiah, Pushpavalli, Ray, Bhadra: "Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines." in: European journal of medicinal chemistry, Vol. 45, Issue 6, pp. 2173-81, 2010 (PubMed).

Boniface, Diveu, Morel, Pedretti, Froger, Ravon, Garcia, Venereau, Preisser, Guignouard, Guillet, Dagregorio, Pène, Moles, Yssel, Chevalier, Bernard, Gascan, Lecron: "Oncostatin M secreted by skin infiltrating T lymphocytes is a potent keratinocyte activator involved in skin inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4615-22, 2007 (PubMed).