ATP5F1 antibody (AA 142-153)
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- Target See all ATP5F1 Antibodies
- ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))
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Binding Specificity
- AA 142-153
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This ATP5F1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- ATP5F1 (aa142-153)
- Sequence
- SIQHIQNAID TE
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product ATP5F1 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 25 kDa band observed in Human Heart lysates (calculated MW of 28.9 kDa according to NP_001679.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))
- Alternative Name
- ATP5F1 (ATP5F1 Products)
- Synonyms
- PIG47 antibody, C76477 antibody, wu:fb59g11 antibody, wu:fj08b08 antibody, zgc:101887 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 S homeolog antibody, ATP synthase F(0) complex subunit B1, mitochondrial antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1 antibody, ATP5F1 antibody, atp5f1.S antibody, atp5f1 antibody, LOC479901 antibody, Atp5f1 antibody
- Background
- ATP5F1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1, PIG47, ATP synthase B chain, mitochondrial, ATP synthase subunit b, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, ATP synthase, H+ transport
- Gene ID
- 515
- NCBI Accession
- NP_001679
- Pathways
- Proton Transport, Ribonucleoside Biosynthetic Process
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