ATP5A1 antibody (AA 83-94)

Catalog No. ABIN2617320

Product Details anti-ATP5A1 Antibody

Target: ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))

Binding Specificity: AA 83-94

Reactivity: Human, Mouse, Rat, Pig, Monkey, Rabbit, Bat, Cow, Hamster, Horse, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP5A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ATP5A1 / ATP Synthase Alpha. This antibody is expected to recognize all reported isoforms (NP_004037.1, NP_001244263.1, NP_001001935.1).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RVHGLRNVQAEE from an internal region (near N-terminus) of human ATP5A1 / ATP Synthase Alpha (NP_004037.1, NP_001244263.1, NP_001001935.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Ferret, Sheep, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Horse, Pig, Platypus (100%), Guinea pig, Chicken, Xenopus, Zebrafish, Sea squirt, Tunicate (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 52kD band observed in Human, Mouse and Rat Heart lysates (calculated MW of 54.5kD according to NP_001001935.1). Recommended concentration: 0.01-0.03 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATP5A1

Target: ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))

Alternative Name: ATP5A1 / ATP Synthase alpha (ATP5A1 Products)

Background: Name/Gene ID: ATP5A1
Subfamily: ATPase - F type
Family: Transporter

Synonyms: ATP5A1, ATP5AL2, ATP5A, HATP1, Mitochondrial ATP synthase, MOM2, ORM, OMR, ATPM

Gene ID: 498

NCBI Accession: NP_004037, NP_001244263, NP_001001935

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process