EGF antibody (Extracellular Domain)
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- Target See all EGF Antibodies
- EGF (Epidermal Growth Factor (EGF))
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Binding Specificity
- AA 106-119, Extracellular Domain
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Reactivity
- Human, Rabbit, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This EGF antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human EGF. This antibody is expected to recognize all reported isoforms (NP_001954.2, NP_001171601.1, NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide SRQERVCNIEKNVS from an internal region (near the N-terminus) of human EGF (NP_001954.2, NP_001171601.1, NP_001171602.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Rabbit (100%), Ferret (93%), Bat, Guinea pig (86%), Rat (57%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product EGF Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 160kD double band observed in Human Prostate lysates (calculated MW of 134kD according to NP_001954.2). The two bands represent glycosylated forms of the immature protein. Recommended concentration: 1-3 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- EGF (Epidermal Growth Factor (EGF))
- Alternative Name
- EGF (EGF Products)
- Synonyms
- HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
- Background
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Name/Gene ID: EGF
Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor - Gene ID
- 1950
- NCBI Accession
- NP_001954, NP_001171601, NP_001171602
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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